Bioprinting Winter School 2024 – From printing set-up to laboratory analysis

The Bioprinting Winter School provides an introduction to the basic principles of 3D (bio)printing, covering both theoretical and practical aspects.

Starting from an overview of the main 3D printing techniques, we proceed to the design of “on-demand” biomaterials suitable for incorporating biological components, and ultimately to the development of a cellular model.

The practical sessions (CAD design, stl export, slicing, bioprinting, and characterization of the printed constructions) will help students solve problems encountered during daily laboratory and research activities.

COURSE LOCATION

The course was held at the University of Pavia, Department of Civil Engineering and Architecture in via Ferrata, 3 – Pavia.

LECTURERS

Michael Gelinsky. Professor and Head of the Centre for Translational Bone, Joint, and Soft Tissue Research, Technische Universitat Dresden, Germany.
Expertise: biomaterials, bioprinting, biofabrication,tissue engineering.

Itedale Namro Redwan. Chief Scientific Officer, Director of Bioink and Tissue Engineering R&D, Cellink, Sweden.
Expertise: Medicinal/Organic Chemistry, Chemical Biology, Stem Cell Biology, Biomaterials, Bioinks and Bioprinting.

Cristina Scielzo. Group Leader, Unit Lymphoid Malignancies, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, Milan, Italy.
Expertise: cell model, 3D in-vitro co-colture system, leukemia microenviroment.

Laura Russo. Associate Professor, School of Medicine, University of Milano-Bicocca, Italy.
Expertise: nano- and biomaterials functionalization for 3D cell culture and medical devices, 3D printed biomaterials for biomedical application, Glycobiomaterials.

Carmelo De Maria.  Associate Professor of Bioengineering at the Dep. Information Engineering, and affiliated with the Research Center “E. Piaggio”, University of Pisa, Italy.
Expertise: additive manufacturing applied to BME, with a particular focus on Bioprinting.

Michele Marino. Associate Professor of Structural Mechanics, Rita-Levi Montalcini Fellow, Alexander von Humboldt Fellow. Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Italy.
Expertise: computational mechanics, constitutive modelling, enabling technologies.

Stefania Marconi. Researcher, Department of Civil Engineering and Architecture, University of Pavia, Italy.
Expertise: biomedical devices, medical image analysis, image segmentation, 3D reconstruction, 3D printing, model post-processing.

Michele Conti. Professor of Industrial Bioengineering, Department of Civil Engineering and Architecture, University of Pavia, Italy.
Expertise: biomechanics, mechanical modeling, bioprinting.

Simona Celi. Senior scientist at Fondazione Toscana G. Monasterio, Massa, Italy.
Expertise: in-silico models through advanced image processing, experimental activities and finite element simulation with uncertainties quantification and sensitivities analysis biomaterials, bioprinting, biofabrication, tissue engineering.

PROGRAM

Day 1: 3D (bio)printing technologies, from segmentation to G-code

• Introduction to the course
• General overview on 3D bioprinting
• Hands-on: From medical images to 3D model

Day 2: Bio-ink & biomaterial

• Development phases of bio-ink
• Rheology and printability
• Hands-on: bioink preparation and printability assessment

Day 3: Cells and read-outs

• How to create a preclinical 3D BioP model
• Read-out strategies
• Design and developments of a bioreactors
• Hands-on: bioinks including cells, read-outs, nanoindentation

Day 4: Numerical simulation

• Numericalsimulatons and bioprinting
• FEA
• Hands-on: FEBio structural assessment of a scaffold and analysis of diffusion of a conpound into 3D bioprinted model

ORGANIZING COMMITTEE

• Michele Conti         michele.conti@unipv.it
• Franca Scocozza    franca.scocozza@unipv.it
• Maria Morello       maria.morello@unipv.it