Bioprinting Winter School 2020 – From printing set-up to laboratory analysis

Bioprinting Winter School provides the basic principles related to 3D (bio)printing both from a theoretical and practical point of view.

Starting from the main 3D printing techniques overview, we move on to the design of “on-demand” biomaterials, suitable for incorporating biological components, up to the development of a cellular model.

The practical sessions (CAD design, stl export, slicing, bioprinting, and characterization of the printed constructions) will help students solve problems encountered during daily laboratory and research activities.



The course was held at the University of Pavia, Department of Civil Engineering and Architecture in via Ferrata, 3 – Pavia.



Cristina Scielzo. Group Leader, Unit Lymphoid Malignancies, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, Milan, Italy. Expertise: cell model, 3D in-vitro co-colture system, leukemia microenviroment.

Giovanni Vozzi. Professor in Bioengineering, Interdepartmental Research Center “E. Piaggio”, University of Pisa, Italy. Expertise: tissue engineering, microfabrication, bioreactors for tissue colture, microactuators fabrication.

Jürgen Groll. Professor, Department for Functional Materials in Medicine and Dentistry,
University of Würzburg, Germany. Expertise: applied polymer chemistry, self-assembly behavior of multifunctional polymer systems, biomimetic scaffolds, biofabrication, immunomodulatory materials and scaffolds, multivalency, nanobiotechnology.

Laura Russo. Researcher, Department of Biotechnology and Bioscience, University of Milano Bicocca, Italy. Expertise: nano- and biomaterials functionalization for 3D cell colture, 3D printed biomaterials for biomedical application.

Stefania Marconi. Researcher, Department of Civil Engineering and Architecture, University of Pavia, Italy. Expertise: biomedical devices, medical image analysis, image segmentation, 3D reconstruction, 3D printing, model post-processing.

Carmelo De Maria. Assistant Professor of Bioengineering at the Dpt. Information Engineering, and affiliated with the Research Center “E. Piaggio”, University of Pisa, Italy. Expertise: additive manufacturing applied to BME, with a particular focus on Bioprinting.

Francesca Vittoria Sbrana. Postdoctoral researcher, IRCCS Ospedale San Raffaele, Milan, Italy. Expertise: Chronic Lymphocytic Leukemia (CLL), specifically the crosstalk between leukemic cells and the microenvironment, by exploiting a bench-top 3D bioprinter.

Matteo Bordoni. Postdoctoral researcher, Genomic and post-Genomic Center, IRCCS Mondino Foundation, Pavia, Italy. Expertise: 3D cell culture, in particular by differentiating human iPSCs into mature neurons in order to generate new in vitro model for the investigation of neurodegenerative disorders.



Day 1: Introduction and 3D printing

  • Introduction to the course, presentation of the program and of the students who will illustrate their projects, the pros and cons related to the field of bioprinting
  • General overview on 3D bioprinting
  • Fundamentals of 3D printing starting from the CAD model to the G-code (theoretical and practical session)

Day 2: Bio-ink & biology

  • Development phases of bio-ink to be used in the bioprinting field starting from the availability of materials, up to the choice of materials based on the application
  • Material characterization and evaluation of the possibility of being processed by 3D bio-plotter
  • Design and development of a cellular disease model in vitro using mainly bioprinting techniques, starting from the generation of a cellular environment as close as possible to the physiological one (printing different types of cells) to laboratory measurements during cell cultures
  • Imaging techniques of biological structures within the bio-ink

Day 3: Hands-on
Practical section in laboratory on the use of bio-plotter and biological analysis tools

On Friday 14 February, the IV workshop will take place in Pavia. According to the school aims, this meeting aims at sharing the experiences gained in the field of bioprinting by highlighting experimental protocols and practical aspects related to the preparation and analysis of 3D printed biological constructs.

Program details